Journal: European Journal of Medical Research

Article Title: Mitochondria-targeted antioxidant mitoquinone mitigates vitrification-induced damage in mouse ovarian tissue by maintaining mitochondrial homeostasis via the p38 MAPK pathway

doi: 10.1186/s40001-024-02181-z

Figure Lengend Snippet: MitoQ improves mitochondrial morphology and homeostasis post-vitrification. A Immunofluorescence staining of ovarian tissues showing the localization of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and anti-Müllerian hormone (AMH), with DAPI co-staining to assess DNA oxidative damage in ovarian tissues from each group (×20 magnification, n = 4). Representative images demonstrate that oxidative damage predominantly occurs in the granulosa cell mitochondria. B Transmission electron microscopy (TEM) images of mitochondrial morphology in ovarian tissues from each group (×4200 magnification, n = 3). Arrows indicate mitochondria. C – E Western blot analysis of mitochondrial dynamics-related proteins Drp1 and Mfn2 in ovarian tissues from each group, with GAPDH as the loading control. Data are presented as mean ± SD (n = 3, ** P < 0.01, *** P < 0.001). Ctr fresh control group, V conventional vitrification group, VQ MitoQ-supplemented vitrification group

Article Snippet: After blocking in 5% BSA, the sections were permeabilized with Triton-X100 (Cat# T8787, Sigma) for 40 min. After rinsing, sections were then incubated with primary antibodies at 4 °C for overnight: 8-OHdG (1:100, Cat# sc-66036, Santa Cruz) and AMH (1:100, Cat#:14461-1-AP, Proteintech).

Techniques: Immunofluorescence, Staining, Transmission Assay, Electron Microscopy, Western Blot, Control